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Sangon Biotech elisa washing buffer
Elisa Washing Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+washing+buffer/buffer+elisa+washing/pm41877479-34-8-21
Average 86 stars, based on 1 article reviews
elisa washing buffer - by Bioz Stars, 2026-09
86/100 stars

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Recombinant:

Article Title: Molecular and serological investigations of Batai virus in cattle and goats in the border area of Yunnan, China (2021–2022)
Article Snippet: After washing three times, an HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (Proteintech, America) was added and incubated at room temperature for 1 h. The membrane was then washed three times and ECL color developing solution was added for imaging. .. The recombinant protein (2.5 μg/mL) was used to coat a 96-well microtiter plate overnight at 4°C and then washed three times in 1× ELISA Washing Buffer (Sangon Biotech Co., Ltd., China) for 5 min per wash according to the manufacturer’s instructions. .. Next, blocking was performed using Blocking Buffer (Sangon Biotech) at 37°C for 1 h, followed by three washes for 5 min per wash.

Enzyme-linked Immunosorbent Assay:

Article Title: Molecular and serological investigations of Batai virus in cattle and goats in the border area of Yunnan, China (2021–2022)
Article Snippet: After washing three times, an HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) (Proteintech, America) was added and incubated at room temperature for 1 h. The membrane was then washed three times and ECL color developing solution was added for imaging. .. The recombinant protein (2.5 μg/mL) was used to coat a 96-well microtiter plate overnight at 4°C and then washed three times in 1× ELISA Washing Buffer (Sangon Biotech Co., Ltd., China) for 5 min per wash according to the manufacturer’s instructions. .. Next, blocking was performed using Blocking Buffer (Sangon Biotech) at 37°C for 1 h, followed by three washes for 5 min per wash.

Article Title: Immunogenicity and efficacy of serogroup A and D bacterins against Pasteurella multocida in mice.
Article Snippet: High-protein-binding 96-well plates (Jet Biofil; TCP011896, Jet Biofil, Guangzhou, China) were coated with 100 μl/well of P. multocida serogroup A or D cells suspended in 0.05M carbonate/bicarbonate buffer (pH 9.6, 100 μl/well) and Frontiers in Veterinary Science 03 frontiersin.org stored overnight at 4◦C. .. The plates were then washed with ELISA washing buffer (Sangon Biotech, E661005, Shanghai, China) and blocked using 15% (wt/vol) skimmed milk powder in PBS for 1 h at 37◦C. ..

Article Title: An Ultrasensitive Single-Particle Collision Electrochemical Immunoassay at Subfemtogram-per-Milliliter Levels via Cascaded Hybridization Chain Reaction Amplification.
Article Snippet: Herein, we developed an ultrasensitive single-particle collision-based electrochemical immunosensing platform for detecting a total prostate-specific antigen (tPSA) as a model target.. It relies on the collision events resulting from the enzymecatalyzed, in situ generated silver shells on gold nanoparticles.. To boost the sensitivity of the method, a branched hybridization chain reaction (bHCR) was employed to introduce abundant alkaline phosphatase (ALP) for signal amplification.

Article Title: Isolation of Elizabethkingia anophelis From COVID-19 Swab Kits
Article Snippet: An ELISA coating buffer (Sangon Biotech, Shanghai, China) was used to dilute the whole bacterial cell antigen to 10 ng/μl, and 100 μl of diluted antigen was added to the 96-well plate (carrier), which was incubated at 37°C for 1 h. The liquid was then discarded and 150 μl of skimmed milk powder (5%) was added to each well. .. Plates were then kept at 37°C for 1 h. Using skimmed milk powder (2%) as the diluent, the patient’s serum was diluted in multiples (range 1:2–1:2,048), and a 100 μl dilution series was added to each test well, and incubated at 37°C for 1.5 h. After washing three times with ELISA Washing Buffer (Sangon Biotech), 100 μl of goat anti-human IgG secondary antibody (horseradish peroxidase-labeled) was added and incubated at 37°C for 40 min. After washing three times with ELISA Washing Buffer, 100 μl ELISA Chromogen Solution (Sangon Biotech) was added and incubated at 37°C for 3 min. .. Finally, 50 μl of ELISA Stopping Solution (Sangon Biotech) was added.

Article Title: Evaluation of the protective efficacy of three novel identified membrane associated proteins of Streptococcus suis serotype 2.
Article Snippet: Streptococcus suis is one of the major pathogens of pigs circulating worldwide, and the development of vaccines will help to effectively control streptococcosis in swine.. In this study, we evaluated the potential of three membrane associated proteins, histidine kinase (HK), glycosyltransferase family 2 (Gtf-2) and phosphate binding protein (PsbP) of S. suis as subunit vaccines.. Bioinformatics analysis shows that protein ABC is highly conserved in S. suis.

other:

Article Title: Isolation of Elizabethkingia anophelis From COVID-19 Swab Kits
Article Snippet: An ELISA coating buffer (Sangon Biotech, Shanghai, China) was used to dilute the whole bacterial cell antigen to 10 ng/μl, and 100 μl of diluted antigen was added to the 96-well plate (carrier), which was incubated at 37°C for 1 h. The liquid was then discarded and 150 μl of skimmed milk powder (5%) was added to each well.

Incubation:

Article Title: Isolation of Elizabethkingia anophelis From COVID-19 Swab Kits
Article Snippet: An ELISA coating buffer (Sangon Biotech, Shanghai, China) was used to dilute the whole bacterial cell antigen to 10 ng/μl, and 100 μl of diluted antigen was added to the 96-well plate (carrier), which was incubated at 37°C for 1 h. The liquid was then discarded and 150 μl of skimmed milk powder (5%) was added to each well. .. Plates were then kept at 37°C for 1 h. Using skimmed milk powder (2%) as the diluent, the patient’s serum was diluted in multiples (range 1:2–1:2,048), and a 100 μl dilution series was added to each test well, and incubated at 37°C for 1.5 h. After washing three times with ELISA Washing Buffer (Sangon Biotech), 100 μl of goat anti-human IgG secondary antibody (horseradish peroxidase-labeled) was added and incubated at 37°C for 40 min. After washing three times with ELISA Washing Buffer, 100 μl ELISA Chromogen Solution (Sangon Biotech) was added and incubated at 37°C for 3 min. .. Finally, 50 μl of ELISA Stopping Solution (Sangon Biotech) was added.



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